Webinar: Automated assessment of Liver and Cardiac toxicities in lead optimisation, using biochemical and human iPS cell assays

22 MAY 2018
​3:00PM (BST)

ABOUT THIS WEBINAR

In this two-part webinar we will cover both automation of P450 (CYP) assays and the automatisation of cellular differentiation processes and compound testing using human induced pluripotent stem cells based assays.  

Metabolism of drugs is a key factor in compound toxicity, and automated assays which monitor inhibition or induction of members of the cytochrome P450 (CYP) superfamily of enzymes are a key ADME readout during lead optimisation. 

In this webinar, we will describe a 384-well microplate format assay using three different CYP enzymes, with two approaches, illustrating a typical assay run in an ADME laboratory. The CYP profiling was performed on the Fluent 780 assay workstation in an automation process designed to reduce reagent consumption and make tip usage as efficient as possible. Prediction of toxicity of small molecule chemicals is essential during drug development and evaluation of industrial chemicals. Human induced pluripotent stem cells (hiPSC) and derived cellular products, e.g. hiPSC-derived heart, liver or brain cells, provide new concepts and possibilities to generate tissues that could be physiologically more relevant and therefore predictable and scalable to sufficient quantities for analysis of toxicological features. The automatisation of cellular differentiation processes and compound testing could enable a stable and scalable solution for toxicity assessment.
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In the second part of the webinar we will cover the adaptation and automatisation of the differentiation process for hiPSC-based cardiomyocytes. The differentiation occurs directly in 96-well format screening plates. Moreover, two endpoint assays – based on ATP detection and intracellular calcium transient visualisation – for the cardiotoxicity assessment were adapted and automated in addition to performing manual beating analysis. As proof-of-concept to evaluate the established platform, inhibitors of the non-selective cation channel TRPM4, which are currently being developed at Fraunhofer IME to target the neurodegenerative disease multiple sclerosis, were tested using the platform technology presented here. We have shown  that hiPSC-cardiomyocytes express TRPM4 after a 14-day differentiation protocol to a similar extend than human cardiomyocytes from explants. The presented automated system therefore appears to be suitable for assessing both toxicity and impacts on functional performance of human cardiomyocytes in early stage drug discovery or similar applications.

LC-MS için örnek hazırlama verimliliğinizi arttırın.

Free webinar at 5 pm CEST on the 11th of April, 2018.

Despite the improvements in the analytical capabilities of today’s mass spectrometers, challenges with sample preparation remain. This webinar introduces the newly launched Resolvex™ A200 automated positive pressure processor for solid phase extraction workflows.

Learn more about how the Resolvex A200 can increase lab productivity by simplifying your sample preparation, using features like integrated fluid dispensing to allow walkaway solid phase extraction. There will also be an opportunity to explore specific applications including proteomics that can benefit from our proprietary smart consumables, saving you time and increasing your process security.
Key benefits

• Automated solid phase extraction to virtually eliminate user to user variability
• Minimized user intervention for increased walkaway time
• Reproducible extraction performance for reliable results

A full recording of his webinar will be available to download after the event.

Free Download: Application Guide for Multimode Readers

Which multimode reader fits best for your application?
The Application Guide summarizes the principles of the most important assays for each detection mode and gives example settings for the readouts.
We are confident that you will find what you are looking for to get your results more quickly.

Bilindiği üzere günümüzde antimikrobiyal direncin giderek artması dünya çapında pek çok ülkede sağlık alanında önemli bir sorun olarak karşımıza çıkmakta.

Antimikrobiyal direnç, antibiyotiklerin aşırı ve yanlış kullanımı ile yanlış bertaraf yöntemleri ile doğal yaşama etkileri gibi önemli birincil nedenlerden oluşan çok çeşitli nedenlerle giderek daha yaygın hale gelmektedir.

Sonuç olarak tedavi yöntemlerindeki başarısızlıklar daha fazla ölüm ve yüksek sosyal maliyetlere neden olmaktadır.

Bu nedenlerle, mikrobiyoloji laboratuvarlarında iş akışını geliştirmek ve numune alım sürecinden dirençlere tanı geliştirilmesine kadar olan süreci içeren yeni metotların geliştirilmesine ihtiyaç duyulmuştur. 

Kütle spektrometresi tabanlı mikrobiyal tanımlama ve antibiyotik hassasiyeti için optimize edilmiş testler umut verici yeni yöntemlerden biridir. Bu yöntemlerde otomasyon kullanımı hataları önlemek ve sonuç kalitesini artırmak için yüksek öneme sahiptir.

Tecan tarafından siz değerli araştırmacılarımıza yönelik olarak klinik mikrobiyoloji alanındaki iş akışına yönelik sunulacak çözümlere ilişkin bir webinar düzenlenecektir. Konu başlıkları kütle spektrometresi numune hazırlama, MALDI spotlama ve veri takibi gibi değişik uygulamalardan oluşmaktadır. 15 Kasım tarihinde Türkiye saati ile 11:00 – 12:00 ve 18:00 – 19:00 saatleri arasında webinar düzenlecektir. Size uygun olan saat dilimindeki oturuma katılımınızdan mutluluk duyarız. Webinarla ilgili detaylı bilgiyi ve katılım için kayıt linkini aşağıda bilgilerinize sunuyoruz.


Clinical Microbiology - Empowered with Tecan
 
Dear Lab Manager
 
A growing emergence of antimicrobial resistance is currently challenging the health-care field in many countries worldwide.
Antimicrobial resistance is becoming more and more relevant due to several reasons, overuse and misuse of antibiotics and their improper disposal into the environment being among the main ones.
The result is an increasing risk for treatment failure, that ultimately leads to more deaths and higher social costs.
 
Therefore, new methods have been developed to improve the workflow in the microbiology laboratories, to help the clinicians in speeding up the process from sample collection through resistance diagnosis to treatment.
Mass spectrometry-based microbial identification and optimized tests for antibiotic susceptibility is one of the promising new methods. Thereby, automation plays a key role to improve the quality of results, to track sample information and to decrease the likelihood of manual error.
 
This webinar will present the automated solutions that Tecan has developed together with specialized partners to streamline the workflow in microbiology labs. Topics will range from automated colony picking to mass spec MALDI spotting, to sample preparation for antibiotic susceptibility testing and data tracking.
 
Register here to get first hand-information on innovative workflow solutions for microbiology processes:

https://attendee.gotowebinar.com/rt/2510681303259304962
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The webinar will take place on

Tuesday November 15th on two times: 9-10am CEST and 4-5pm CEST.
 
Both times are options in the registration drop-down list in the link above. Both webinars will be identical.
 
We welcome you to this webinar.

Tecan tarafından siz değerli araştırmacılarımıza yönelik olarak hücre kültürü biyolojisindeki çalışma prosedürleri temel alınarak; 2D ve 3D hücre kültürü, hücre ayırımı ve transfeksiyonu, hücre toksisitesi ve fenotipik tarama konularında 22 Kasım tarihinde Türkiye saati ile 11:00 – 12:00 ve 18:00 – 19:00 saatleri arasında webinar düzenlecektir. Size uygun olan saat dilimindeki oturuma katılımınızdan mutluluk duyarız. Webinarla ilgili detaylı bilgiyi ve katılım için kayıt linkini aşağıda bilgilerinize sunuyoruz.

Dear Researcher
 
There is currently a trend towards providing an “enriched” understanding of the biological processes involved in every aspect of cell and tissue biology.
Regardless if it is for the  understanding of the mode-of-action of a drug or an ex vivo study for personalized medicine treatment, the use of cell-based assays to ensure greater biological relevance is becoming the new standard.
 
A cell based assay helps to provide a better understanding of how biochemical activity translates into in vitro efficacy and, combined with early
research toxicity testing, which compounds have the best chances for subsequent drug development.
 
An ex vivo study provide immediate, phenotypic, biologically relevant data that combined with genetic and clinical evidences are crucial for subsequent in vivo treatments
 
As such, there is an evident need for new technologies which allow the generation of reliable, high quality biologically relevant data.
 
This webinar showcases a range of technologies, applications, products and workflow solutions which will bring more robustness to your data.
It will cover topics related to: 2D&3Dcell culture, cell separation and transfection, cell toxicity and phenotypic screening.
 
Register here to get first hand-information on innovative workflow solutions for cell biology processes:

https://attendee.gotowebinar.com/rt/1375964413406482946
 
The webinar will take place on November 22th on two times: 9-10am CEST and 4-5pm CEST.
Both times are options in the registration drop-down list in the link above.
 
We welcome you to this webinar